In vivo Pharmacokinetic Analysis of Shenbai Nanosuspension in Rats Based on Overall Composition of Chinese Materia Medica / 中国实验方剂学杂志

ObjectiveTo clarify the scientific validity of in vivo pharmacokinetic determination of the whole drug composition in Shenbai nanosuspension in rats， and to provide methodological guidance and theoretical basis for the in vivo study of multi-component complex system of traditional Chinese medicine（TCM） preparations. MethodThe concentration of the overall components， mainly total saponins and total polysaccharides in Shenbai decoction and Shenbai nanosuspension， was determined in rat plasma at different times by area under the absorbance-wavelength curve method（AUAWC）， and the concentration of individual ginsenoside Rg1 was determined by high performance liquid chromatography（HPLC）， and the methodology was verified. The pharmacokinetic parameters of the whole component were compared with those of ginsenoside Rg1 to evaluate the in vivo operational characteristics of the two preparations. ResultThe methodological investigations of AUAWC and HPLC were in accordance with the requirements. AUAWC analysis showed that the overall components in both the decoction group and the nanosuspension group showed a one-compartment model， with half-life（t1/2） of 2.43 h and 2.04 h， respectively. The relative bioavailability of Shenbai nanosuspension was 138.99%. HPLC assay showed that ginsenoside Rg1 in the decoction group and the nanosuspension group showed a two-compartment model， with distribution half-life（t1/2α） of 0.13 h and 2.55 h， and elimination half-life（t1/2β） were 14.28 h and 3.85 h， respectively. The relative bioavailability of Shenbai nanosuspension was 127.49%. Compared with Shenbai decoction， the time to peak（tmax）， peak concentration（Cmax） and area under the drug-time curve（AUC） of the overall components and ginsenoside Rg1 in Shenbai nanosuspension were increased. ConclusionThe established AUAWC can be used for the pharmacokinetic study of the overall components of TCM preparations， which is complementary to the results of individual components measured by HPLC， and can provide useful reference for the in vivo study of new dosage forms of TCM.

Estrategias para la evaluación de extractos de polifenoles en modelos in vitro de cáncer de vías digestivas / Strategies for Evaluation of Polyphenol Extracts on in vitro Models of Digestive Tract Cancer / Estratégias para a avaliação de extratos de polifenóis em modelos in vitro de câncer do sistema digestivo

Introducción: Los polifenoles son compuestos que se encuentran naturalmente en alimentos como frutas, verduras, té, vino y chocolates, a los que se les atribuyen beneficios a la salud humana por su capacidad antioxidante. El cáncer de las vías digestivas se encuentra entre la tercera y quinta causas de muerte para la población, por lo que se ha incrementado el interés por realizar los estudios dirigidos a encontrar compuestos polifenólicos que ayuden en su prevención o tratamiento. Objetivo: Identificar las estrategias disponibles para la evaluación de polifenoles en células de cáncer de vías digestivas. Metodología: Búsqueda de literatura en bases de datos como Ovid, Pubmed, Science Direct y Elsevier Journal. Se seleccionaron artículos en los cuales se reporta el efecto biológico de los polifenoles sobre líneas celulares de cáncer de vías digestivas publicados entre 2012 y 2022. Resultados: Varios estudios han reportado el uso de un buen número de líneas celulares como modelos in vitropara estudios de polifenoles en cáncer y han resaltado las líneas AGS y HT-29, además de técnicas para la caracterización de los polifenoles, como el ensayo 2,2-Difenil-I-Picril Hidrazilo (DPPH). Sin embargo, para evaluar el efecto biológico se identifican diversas pruebas que deben analizarse antes de su implementación. Conclusiones: En la literatura se identifica que existen varias alternativas y estrategias para la evaluación de extrac-tos vegetales en cultivos in vitro de cáncer de vías digestivas; no obstante, antes de pasar al diseño experimental, deben tenerse en cuenta una serie de consideraciones para garantizar la utilidad de los resultados.

Introduction: Polyphenols are compounds naturally found in foods such as fruits, vegetables, tea, wine and chocolates, and it was attributed with benefits to human health due to their antioxidant capacity. Cancer of the digestive tract is between the third and fifth cause of death for the population, increasing the interest in carrying out studies aimed at finding polyphenolic compounds that help in their prevention or treatment. Objective: Identify the available strategies for the evaluation of polyphenols in digestive tract cancer cells. Method: A literature search was performed in databases such Ovid, Pubmed, Science Direct and Elsevier Journal and selected articles reporting the biological effect of polyphenols on digestive tract cancer cell lines, published between 2012 and 2022. Results: Currently studies report the use of a good number of cell lines as in vitro models for poly-phenol studies in cancer highlighting the AGS and HT-29 lines, in addition to techniques for the characterization of polyphenols such as the α, α-diphenyl-ß-picrylhydrazyl DPPH assay, however, to evaluate the biological effect various tests are identified that should be analyzed before implemen-tation. Conclusions: The literature identifies that there are many alternatives and strategies for the evaluation of plant extracts in in vitro cultures of digestive tract cancer, however, before moving on to the experimental design, a number of considerations should be taken into account to ensure the usability of the results

Introdução: Os polifenóis são compostos encontrados naturalmente em alimentos como frutas, legumes, chá, vinho e chocolates, aos quais são atribuídos benefícios para a saúde humana devido à sua capacidade antioxidante. O câncer do sistema digestivo está entre a terceira e a quinta principais causas de morte na população, o que levou a um interesse crescente em estudos destinados a encon-trar compostos polifenólicos que ajudem a prevenir ou tratar esse tipo de câncer. Objetivo: Identificar as estratégias disponíveis para a avaliação dos polifenóis nas células cancerosas do sistema digestivo. Metodologia: Pesquisa bibliográfica em bases de dados como Ovid, Pubmed, Science Direct e Elsevier Journal. Foram selecionados artigos que relatam o efeito biológico dos polifenóis em linhas celulares de câncer do sistema digestivo, publicados entre 2012 e 2022. Resultados: Vários estudos relataram a utilização de várias linhas celulares como modelos in vitro para estudos de polifenóis no câncer destacando as linhas AGS e HT-29, bem como técnicas para a ca-racterização de polifenóis, como o ensaio 2,2-Difenil-I-Picril Hidrazil (DPPH). No entanto, para avaliar o efeito biológico, são identificados vários testes que devem ser analisados antes da sua aplicação. Conclusões: A literatura identifica que existem várias alternativas e estratégias para a avaliação de extratos de plantas em culturas in vitro de câncer do sistema digestivo; no entanto, antes de passar à concepção experimental, é necessário ter em conta uma série de considerações para garantir a uti-lidade dos resultados

Smart Spectrophotometric Methods for Concurrent Determination of Furosemide and Spironolactone Mixture in Their Pharmaceutical Dosage Forms

Abstract Simple, precise, accurate and specific spectrophotometric methods are progressed and validated for concurrent analysis of Furosemide (FUR) and Spironolactone (SPR) in their combined dosage form depend on spectral analysis procedures. Furosemide (FUR) in the binary mixture could be analyzed at its λmax 274 nm using its recovered zero order absorption spectrum using constant multiplication method (CM). Spironolactone (SPR) in the mixture could be analyzed at its λmax 238 nm by ratio subtraction method (RS). Concurrent determination for FUR and SPR in their mixture could be applied by amplitude modulation method (AM), absorbance subtraction method (AS) and ratio difference (RD). Linearity ranges of FUR and SPR were (2.0µg/mL-22.0 µg/mL) and (3.0µg/mL-30.0 µg/mL), respectively. Specificity of the proposed spectrophotometric methods was examined by analyzing the prepared mixtures in laboratory and was applied successfully for pharmaceutical dosage form analysis which have the cited drugs without additives contribution. The proposed spectrophotometric methods were also validated as per as the guidelines of ICH. Statistical comparison was performed between the obtained results with those from the official methods of the cited drugs, using one-way ANOVA, F-test and student t-test. The results are exhibiting insignificant difference concerning precision and accuracy

Numerical study on the effect of middle ear malformations on energy absorbance / 生物医学工程学杂志

In order to study the effect of middle ear malformations on energy absorbance, we constructed a mechanical model that can simulate the energy absorbance of the human ear based on our previous human ear finite element model. The validation of this model was confirmed by two sets of experimental data. Based on this model, three common types of middle ear malformations,

A Novel Spectrophotometric and RP-HPLC methods for Determination of Nortriptyline hydrochloride and Pregabalin in Tablets

A Simple, rapid, specific, accurate, economical and precise UV spectrophotometric and RP-HPLC methods (inaccordance with ICH guidelines) were developed and validated for determination of Nortriptyline hydrochlorideand Pregabalin in tablet dosage form. The first method was based on Q - absorbance ratio, and absorbances ofboth drugs were determined at 239 nm (λmax of Nortriptyline Hydrochloride) and 235 nm (Iso-absorptive Point)when dissolved in methanol. It is found that Pregabalin does not have chromophoric group. To be UV-sensitive,it was compulsory to introduce chromophoric group in Pregabalin structure and make it UV-sensitive. This wasachieved by converting the primary amine group of Pregabalin through reaction with benzoyl chloride to formbenzoylated derivative of Pregabalin. Benzoylated Pregabalin was determined at 225 nm using UV-visiblespectrophotometer. The second method was based on RP-HPLC. The chromatographic separation was performedon an Inertsil ODS C18 column (250 x 4.6mmx 5 μm) with a mobile phase of 0.56 %w/v Sodium hexane sulphonicacid dissolved in water acetonitrile (50:50 %v/v, pH 4.5 adjusted with Glacial Acetic Acid) at flow rate of 1.0mL/min with DAD detection wavelength at 210 nm. Retention times of Nortriptyline Hydrochloride andPregabalin were 7.3894 min and 4.0506 min, respectively. Beer-Lambert’s law obeyed the concentration rangeof 2-12 μg/mL for Nortriptyline Hydrochloride and 10-60 μg/mL for Pregabalin. The results indicated that bothspectrophotometric and RP-HPLC methods were linear, accurate, precise and robust with RSD values less than0.2% and % recovery was within the standard limits (99 - 102%).

Bioengineered miR-328-3p modulates GLUT1-mediated glucose uptake and metabolism to exert synergistic antiproliferative effects with chemotherapeutics

MicroRNAs (miRNAs or miRs) are small noncoding RNAs derived from genome to control target gene expression. Recently we have developed a novel platform permitting high-yield production of bioengineered miRNA agents (BERA). This study is to produce and utilize novel fully-humanized BERA/miR-328-3p molecule (hBERA/miR-328) to delineate the role of miR-328-3p in controlling nutrient uptake essential for cell metabolism. We first demonstrated successful high-level expression of hBERA/miR-328 in bacteria and purification to high degree of homogeneity (>98%). Biologic miR-328-3p prodrug was selectively processed to miR-328-3p to suppress the growth of highly-proliferative human osteosarcoma (OS) cells. Besides glucose transporter protein type 1, gene symbol solute carrier family 2 member 1 (GLUT1/), we identified and verified large neutral amino acid transporter 1, gene symbol solute carrier family 7 member 5 (LAT1/) as a direct target for miR-328-3p. While reduction of LAT1 protein levels by miR-328-3p did not alter homeostasis of amino acids within OS cells, suppression of GLUT1 led to a significantly lower glucose uptake and decline in intracellular levels of glucose and glycolytic metabolite lactate. Moreover, combination treatment with hBERA/miR-328 and cisplatin or doxorubicin exerted a strong synergism in the inhibition of OS cell proliferation. These findings support the utility of novel bioengineered RNA molecules and establish an important role of miR-328-3p in the control of nutrient transport and homeostasis behind cancer metabolism.

Microscopio como Lector de Absorbancia con Utilidad en Análisis Clínicos / Microscope as Absorbance Reader with Utility in Clinical Analysis

Resumen La descentralización de laboratorios clínicos, se encuentra en desarrollo, lo anterior ha llevado a diseñar instrumentos que ofrecen resultados rápidos, confiables y al lado del paciente, esta tendencia se le conoce como prueba en el punto de atención (point of core testing POCT) y brinda la posibilidad de dar un seguimiento inmediato al padecimiento. El objetivo de esta investigación fue la implementación de un medidor de absorbancia, empleando la estructura de un microscopio óptico, al cual se le ha adaptado un filtro de luz, y una cámara digital. Lo anterior permite obtener valores de intensidad promedio de imágenes sólidas microscópicas de reacciones enzimáticas, y a partir de ellas estimar absorbancia o concentración. Como resultados se presentan rectas de calibración de absorbancia y mediciones de concentraciones para los casos de violeta de genciana, un kit de glucosa oxidasa y muestras problemas de pacientes voluntarios. Concluimos que existe un error de medición menor de ±1mg/dL comparados con las mediciones de un lector de placas de Elisa y un analizador de química sanguínea semiautomatizado. Teniendo en cuenta lo anterior el sistema resulta ser una alternativa viable para la estimación de absorbancia y aumenta la funcionalidad de microscopios ópticos en clínicas de salud.

Abstract The decentralization of clinical laboratories is under development, which has led to the design of instruments that offer fast, reliable and patient-side results, this trend is known as point of core testing (POCT) and It offers the possibility of giving an immediate follow-up to the disease. The objective of this investigation was the implementation of an absorbance meter, using the structure of an optical microscope, to which a light filter and a digital camera have been adapted. This allows to obtain values of average intensity of solid images of enzymatic reactions, and from them to estimate absorbance or concentration. As results, we present absorbance calibration lines and concentration measurements for cases of gentian violet, a glucose oxidase kit and samples of volunteer patients. We conclude that there is a measurement error of less than ± 1mg / dl compared with the measurements of an Elisa plate reader and a semi-automated blood chemistry analyzer. Taking into account the above, the system turns out to be a viable alternative for estimating absorbance and increasing the functionality of optical microscopes in health clinics.

Bleaching of stained resin using nitrogen doped-TiO₂ nanoparticles / 대한치과재료학회지

There has been increasing use of the H₂O₂-based teeth bleaching agents. The purpose of this study was to evaluate the bleaching effectiveness of the laser irradiation combined with nitrogen doped-TiO₂ nanoparticles (NPs) on the stained resin. Nitrogen (N) doped-TiO₂ NPs were prepared under sol-gel method. Light absorbance, X-ray diffraction patterns of NPs, and bleaching of methylene blue and stained resins were evaluated. For bleaching of stained resin, NPs-containing gel was used. For irradiation, light of two different wavelengths was used. Unlike TiO₂, N-TiO₂ showed high absorbance after 400 nm. N-TiO₂, which have used TiN as a precursor, showed a new rutile phase at the TiN structure. For methylene blue solution, N-TiO₂ with 3% H₂O₂ resulted in the greatest absorbance decrease after laser irradiation regardless of wavelength. For stained resin test, N-TiO₂ with 3% H₂O₂ resulted in the greatest color difference after laser irradiation, followed by group that used N-TiO₂ without 3% H₂O₂.

Analysis of the Absorbance Pattern of Postmortem Blood Sample Using Spectrometer / 대한법의학회지

The diagnosis of cause of death (COD) or estimation of postmortem interval (PMI) is hard to perform using postmortem blood or other bodily fluids due to various biochemical changes that occur during the agonal phase or after death. To solve these problems, new paradigms and new analytical methods are needed. In this study, postmortem blood was fractionated with specific gravity 1.021, 1.029, 1.038, and 1.045, and the absorbance patterns of each sample of the 131 total cases (12 kinds of COD) were analyzed using a spectrometer. The absorbance was grouped into 9 patterns (ABS pattern 1 to 9) according to the wave length and the signal intensity. These patterns of postmortem blood were found to be distinctly different from the absorbance pattern of fresh blood. The analysis of ABS patterns is useful for the diagnosis of deaths due to acute or rapid bleeding, fire death, drowning and, in some cases, poisoning, but is not useful for the estimation of PMI.

The Effect of Ethnicity on Wideband Absorbance of Neonates with Healthy Middle Ear Functions in Malaysia: A Preliminary Study

BACKGROUND AND OBJECTIVES: Although ethnicity effect on wideband absorbance (WBA) findings was evident for adults, its effect on neonates has not been established yet. This study aimed to investigate the influence of ethnicity on WBA measured at 0 daPa from neonates with healthy middle ear functions. SUBJECTS AND METHODS: Participants were 99 normal, healthy, full-term newborn babies with chronological age between 11 and 128 hours of age (mean=46.73, standard deviation=26.36). A cross-sectional study design was used to measure WBA at 16 one-third octave frequency points from 99 neonates comprising of three ethnic groups: Malays (n=58), Chinese (n=13) and Indians (n=28). A total of 165 ears (83.3%) that passed a battery of tests involving distortion product otoacoustic emissions, 1 kHz tympanometry and acoustic stapedial reflex were further tested using WBA. Moreover, body size measurements were recorded from each participant. RESULTS: The Malays and Indians neonates showed almost identical WBA response across the frequency range while the Chinese babies showed lower absorbance values between 1.25 kHz and 5 kHz. However, the differences observed in WBA between the three ethnic groups were not statistically significant (p=0.23). Additionally, there were no statistically significant difference in birth weight, height and head circumference among the three ethnic groups. CONCLUSIONS: This study showed that Malays, Chinese and Indians neonates were not significantly different in their WBA responses. In conclusion, to apply for the ethnic-specific norms is not warranted when testing neonates from population constitute of these three ethnicities.

Seroprevalencia de la enfermedad de Chagas en Aguarico, provincia de Orellana. Selva amazónica ecuatoriana 2008- 2015 / Seroprevalence of Chagas disease in Aguarico, Orellana province. Ecuadorian rainforest 2008-2015

Objetivo: Determinar la seroprevalencia de la enfermedad de Chagas del cantón Aguarico, provincia de Orellana. Selva amazónica ecuatoriana (2008-2015). Introducción: En el cantón Aguarico los primeros estudios investigativos sobre esta patología comprenden desde 1990 con 1.011 examinados y 61 positivos (3,40 %), 2003 con 6.866 examinados y 76 reactivos (2,40%), 2009 con una población de 2.083 pacientes con 73 reactivos, (3,60 %) y 2008-2015 con 2.235 examinados y 76 positivos (3,40 %) (presente investigación). Material y métodos: Se procesaron las muestras con la técnica Chagatest/Elisa recombinante v.3.0 para determinar positividad a la enfermedad de Chagas. Los casos positivos fueron reconfirmados por el INSPI, Instituto Nacional de Salud Pública e Investigación, con pruebas de hemoaglutinación indirecta (HAI) y Elisa. Con una absorbancia mayor a 1.300. Resultados: La seroprevalencia en las comunidades los porcentajes oscilaron entre (1,32 %) y (13,16 %). Con mayor frecuencia en el sexo femenino (60,53 %). El Riesgo Relativo (RR) de 1,17. El Odds Ratio (OR) 1,18. Siendo mayor el riesgo a desarrollar la enfermedad en los expuestos que en los no expuestos. Conclusiones: Los datos obtenidos confirman la presencia de un foco autóctono de la enfermedad de Chagas en la selva amazónica ecuatoriana. No se detectaron cuadros clínicos agudos ni patologías crónicas, al momento del estudio sin alteraciones radiológicas y con resultados de electrocardiogramas normales; se concluye que la presente investigación, los casos reactivos corresponderían a enfermedad de Chagas en fase indeterminada.

Objective: To determine the seroprevalence of Chagas disease in canton Aguarico Ecuadorian, Province of Orellana. Amazon Forest (2008-2015).Background: The first research studies on this pathology did in Canton Aguarico since 1990, have included 1.011 examined 61 positives (3,40 %), 2003 2.003 with 6866 examined and 76 reactive (2,40 %), 2009 with a population of 2.083 73 reagents, 3,60 %) and 2008-2015 with 2.235 examined and 76 positives (3,40 %) (Present research). Material and methods: the samples were processed using recombinant CHAGATEST / ELISA technique v.3.0 to determine the positivity to Chagas' disease. The National Institute of Public Health and Research (INSPI) reconfirmed positive cases with indirect hemagglutination (HAI) and ELISA tests. With an absorbance greater than 1300. Results: The sero-prevalence in the communities with percentages ranged from 1 (1,32 %) to 10 (13,16 %), often the greater frequency in females. 46 (60,53 %). The Relative Risk (RR) of 1.17. The Odds Ratio (OR) 1.18, being greater the risk to develop the disease in the exposed than in the unexposed ones. Conclusions: The data obtained confirm the presence of an indigenous provenance of Chagas' disease in the Ecuadorian Amazon Forest. There were not detected any acute clinical cases or chronic pathology at the time of the study, withoutradiological alterations and normal electrocardiogram; It is concluded that the present investigation, the reactive cases would correspond to Chagas disease in Indeterminate phase.

Discoloration of wastewater from a paint industry by the microalgae Chlorella sp / Decoloración de aguas residuales de una industria de pinturas por la microalga Chlorella sp

ABSTRACT Objective. Decoloring wastewater from a paint factory making use ofChlorella sp., microalgae as a biological way of treatment. Materials and methods. Samples of this microalgae previously cultivated with nourishing fertilizer under photoperiods of light and darkness were taken to test the microalgae Chlorella sp., initial concentration effect in the bioremoval process. For this purpose, it was cultivated in 0.10, 0.20 and 0.30 units of absorbance in bioreactors with 200 mL wastewater with and without nutrients. The biotest with the best rate of colour removal was chosen and the DBO5 and DQO were marked out. The immobilized Chlorella sp., in kappa carrageenan was also tested. Results. In the tests colour decrease percentage were 81.7, 69.7 and 58.3% without nutrients in the initial concentrations of 0.10, 0.20 and 0.30 units of absorbance respectively and 72.6, 69.0 and 86.8% for 0.10, 0.20 and 0.30 units of absorbance with nutrients respectively in the day of maximum growth. The immobilized microalgae score were 72.60% and 78.36% of color removal for 0.4 and 1.6 units of absorbance respectively. The higher colour removal test score was that with nutrients at 0.30 units of absorbance with several changes in DBO5 and DQO values. Conclusion. The biological wastewater treatment making use of Chlorella sp., microalgae can be considered as an effective choice in decolorating wastewater.

RESUMEN Objetivo. Decolorar aguas residuales obtenidas de una empresa de pinturas, empleando la microalga Chlorella sp., como medio biológico de tratamiento. Materiales y métodos. Muestras de la microalga previamente cultivada con fertilizante como nutriente y fotoperiodos de luz y oscuridad, se tomaron para evaluar el efecto de la concentración inicial de la microalga Chlorella sp., en el proceso de bioremoción. Para tal fin, esta se cultivó a 0.10, 0.20 y 0.30 unidades de absorbancia en biorreactores con 200 mL de aguas residuales en presencia y ausencia de nutrientes. Se seleccionó el bioensayo con mejores porcentajes de remoción del color y se le determinó el DBO5 y DQO. Chlorella sp., inmovilizada en kappa carragenina también se estudió. Resultados. Los porcentajes de reducción de color de los bioensayos en ausencia de nutrientes fueron de 81.7, 69.7 y 58.3% para las concentraciones iniciales de 0.10, 0.20 y 0.30 unidades de absorbancia respectivamente y en presencia de nutrientes fueron 72.6, 69.0 y 86.8% para 0.10, 0.20 y 0.30 unidades de absorbancia respectivamente, en el día de máximo crecimiento. Los resultados de la microalga inmovilizada fueron de 72.60% y 78.36% de remoción del color para 0.4 y 1.6 unidades de absorbancia respectivamente. El bioensayo con mayor rendimiento de remoción fue el realizado en presencia de nutrientes a 0.30 unidades de absorbancia con cambios importantes en los valores de DBO5 y DQO. Conclusión. El tratamiento biológico de aguas empleando la microalga Chlorella sp., puede considerarse una alternativa eficaz en la decoloración de aguas residuales.

Study on the micelle properties of montelukast sodium in aqueous solution / 药学实践杂志

Objective To study the physicochemical properties of aqueous solution of surface active drug montelukast so-dium (MS) ,which could provide experimental basis for further development of micelle or mixed micelle preparations .Methods Critical micelle concentration (CMC) of MS at different temperatures were determined by conductivity measurements .The absorbance and transmittance of MS aqueous solution were measured by UV at different sodium chloride concentration levels . The micelle stability was evaluated via high speed centrifugal .Results The CMC of MS aqueous solution at 25℃ ,30℃ ,35℃were 0 .75 ,0 .82 ,0 .90 mmol/L .The absorbance and transmittance of MS aqueous solution were affected by the sodium chlo-ride concentration and the concentration of MS itself .It was observed that a clear solution was obtained when MS concentration>7 .5 mmol/L and no precipitation was noticed even after high speed centrifugal .Conclusion Montelukast sodium is a surface active drug .Its solubility is related to MS concentration .The solubility is also sensitive to the temperature and the electrolyte concentration .These unique physicochemical properties could be used to develop micelle or mixed micelle pharmaceutical prepa-rations .

Wideband Absorbance Measurements: Norms and Middle Ear Disorders / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: To evaluate the usefulness of wideband tympanometry in predicting middle ear disorders by comparing the absorbance measurements of the disorder and the norm. SUBJECTS AND METHOD: Wideband tympanometry, pure tone audiometry, and endoscopic examination were performed on 284 ears of 190 subjects. Wideband absorbance (WBA) was measured from normal ears (adults: 128 ears, children: 32 ears), disorders of middle ear included effusion (adults: 24 ears, children: 21 ears), tympanic membrane (TM) retraction (adults: 26 ears, children: 6 ears) and perforation (47 adult ears). RESULTS: The normative data of WBA were similar to previous reports from other countries. WBA of the 17-29 age group showed higher values than other age groups at 1600-3150 Hz. Men had higher absorbance at low frequencies whereas women had higher absorbance at high frequencies. There was a significant gender difference at 4000 Hz. Adults showed higher absorbance at 1000, 1250, and 1600 Hz and lower absorbance at 2500, 3150, and 4000 Hz than children did at those respective frequencies. Compared to the normal group, WBA was decreased at all frequencies by more than 500 Hz in ears with middle ear effusion and at most frequencies by less than 2000 Hz in ear with TM retraction. TM perforation showed higher absorbance in low frequencies; the absorbance decreased as the size of perforation increased. CONCLUSION: The present study established normative WBA data and the results showed comparable statistics to former studies in age and gender difference. WBA provided a high reliability in discrimination of middle ear effusion and TM retraction. WBA is a simple, non-invasive and useful diagnostic tool for middle ear disease.

Analytical method development and validation for simultaneous estimation of Teneligliptin hydrobromide hydrate and Metformin hydrochloride from it’s pharmaceutical dosage form by three different UV spectrophotometric methods.

Three new UV spectrophotometric methods namely simultaneous equation, absorbance ratio and first derivative (zero crossing) spectroscopic methods were developed and validated for simultaneous estimation of teneligliptin hydrobromide hydrate and metformin hydrochloride in tablet formulation which were simple, sensitive, precise and accurate. In simultaneous equation method, absorbance was measured at 237 and 246 nm for both the drugs. Teneligliptin hydrobromide hydrate and metformin hydrochloride was estimated using 237 and 247.5 nm in absorbance ratio method. First derivative (zero crossing) method was based on the transformation of UV spectra in to first derivative spectra followed by measurement of first derivative signal at 237 and 246 nm for teneligliptin hydrobromide hydrate and metformin hydrochloride, respectively using 2 nm as wavelength interval (Δλ) and 1 as scaling factor. Developed methods were validated according to ICH guidelines including parameters viz., specificity, linearity and range, precision, accuracy, limit of detection and quantification. All the three methods showed linear response in the concentration range of 1-20 µg/ml for both the drugs. Results of method validation parameters follows ICH guideline acceptable limits. Based on the assay results obtained, methods were compared using one-way ANOVA followed by Bonferroni multiple comparison tests (95% confidence level) using computer based fitting program (Prism, Graphpad version 5, Graphpad Software Inc). Outcome of the statistical analysis proved that there was no considerable dissimilarity between all the developed methods. Methods were found to be simple, fast, highly sensitive, cost effective and hence can be useful for simultaneous estimation of teneligliptin hydrobromide hydrate and metformin hydrochloride in commercial tablet formulation for routine quality control analysis.

Simultaneous estimation of Aliskiren hemifumarate and Hydrochlorothiazide in combined Tablet Formulation by Simultaneous equation, Absorbance ratio and First derivative Spectroscopic Methods.

Three simple, sensitive, precise and accurate UV-spectroscopic methods namely simultaneous equation, absorbance ratio and first derivative (zero crossing) spectroscopic methods were developed and validated for simultaneous determination of aliskiren hemifumarate and hydrochlorothiazide in tablet dosage form. Simultaneous equation method was based on the measurement of absorbance at 271 and 280 nm for both the drugs. In absorbance ratio method 255 and 271 nm was used for the quantification of aliskiren hemifumarate and hydrochlorothiazide. First derivative method was involved in the conversion of UV-spectra in to first derivative spectra and measurement of first derivative signal at 241 and 280.2 nm for aliskiren hemifumarate and hydrochlorothiazide, respectively using 2 nm as wavelength interval (Δλ) and 1 as scaling factor. Methods were validated as per ICH guidelines including parameters viz., specificity, linearity and range, precision, accuracy, limit of detection and quantification. All the methods were found to be linear in the concentration range of 6-300 μg/ml for aliskiren hemifumarate and 0.5-25 μg/ml for hydrochlorothiazide. Results of validation studies follows ICH guideline acceptable limits. Methods were compared based on the assay results obtained using one-way ANOVA followed by Bonferroni multiple comparison tests (95% confidence level) as appropriate using computer based fitting program (Prism, Graphpad version 5, Graphpad Software Inc). Results of statistical analysis revealed that there was no significant difference between simultaneous equation, absorbance ratio and first derivative method. Developed methods were simple, rapid, highly sensitive and cost effective as compared to existing methods and can be useful for simultaneous estimation of aliskiren hemifumarate and hydrochlorothiazide in commercial tablet formulation for routine quality control.

Construction of NIRS in-line rapid detection model of concentration process of Artemisiae Annuae Herba / 中草药

Objective: In this study, an in-line near infrared spectroscopy (NIRS) rapid detection model of single-effect concentration process of ArtemisiaeAnnuae Herba(AAH) was established and the impact of absorbance to model was discussed. Methods: Nine batches of concentration process samples were collected inline and partial least square (PLS) method was applied to build its quantitative model which was used to predict an unknown batch of samples. Results: The parameters of PLS model of total acid and soluble solid content were as follows: coefficient of determination (R2) of 0.9623 and 0.9679, root mean square errors of calibration (RMSEC) of 0.7835 and 0.9488, and root mean square errors of cross-validation (RMSECV) of 0.8258 and 0.9780, respectively. Conclusion: Absorbance of concentration process samples of AAH was between 0 and 2.0 and RSEP values of total acid and soluble solid content was less than 10%. It can meet the quality requirements of production process of AAH and indicate that the effect of high absorbance (namely low tansmittance) on NIRS model of AAH can be eliminated by adding samples and using chemometrics when the absorbance is between 0.0 and 2.0.

Antioxidant potential, tannin and polyphenol contents of seed and pericarp of three Coffea species

OBJECTIVE@#To investigate the antioxidant activity, total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis (C. benghalensis) and Coffea liberica compared to Coffea arabica (C. arabica).@*METHODS@#The antioxidant potential, total tannin and polyphenol contents of the immature and mature seed and pericarp of C. benghalensis and Coffea liberica were quantified and compared to C. arabica. Enhanced chemiluminescence (ECL), 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity, Folin-Ciocalteau method and total tannin content assays were used.@*RESULTS@#Trolox equivalent (TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation (r(2) = 0.587) while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant. A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique (r(2) = 0.610 7) in each species, however the immature pericarp of C. benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay. The immature pericarp of C. benghalensis expressed the highest tannin and polyphenol content, and a high polyphenol level was also detected in the immature seed of C. arabica. The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents.@*CONCLUSIONS@#The obtained data highlight the potential role of C. benghalensis as a new source of natural antioxidants and polyphenols compared to C. arabica.

Effects of Tussilago farfara by Grinding Dispersion Treatment on the Dispersion Stability of Xiao’er Feike Granules in Water / 中国药房

OBJECTIVE:To improve the dispersion stability in water of Tussilago farfara powder,and to improve compliance of Xiao’er feike granules. METHODS:The effects of 4 kinds of dispersion stabilizer (sodium hexametaphosphate, dextrin, PEG4000 and lecithin) on dispersion stability of suspension in water were investigated during the grinding of T. farfara using rate of absorbance change(β)and Zeta potential as index;IR spectrum of samples were characterized. Using original formulation with-out dispersion stabilizer as control,the dispersion stability of new formulation granules in water were analyzed comparatively after adding dispersion stabilizer. RESULTS:Among 4 kinds of dispersion stabilizer,β of sample prepared by sodium hexametaphos-phate was the lowest,while Zeta potential of it was the highest;compared with original T. farfara,β of T. farfara grinded with 2.5% sodium hexametaphosphate decreased by 16.8%,and Zeta potential absolute value increased by 29.4%;no new peak was found in IR spectrum. Compared with control granules,granules suspension prepared by new formulation had lower β and higher Zeta potential absolute value (P<0.01);particle size was 30 μm and no large particle aggregation was found;β was less than 5.0% within 20 s sedimentation. CONCLUSIONS:During the preparation of Xiao’er feike granules,the application of sodium hexametaphosphate in the grinding of T. farfara powder can improve the dispersion stability of granules in water and the compliance of the preparation.

Antioxidant potential, tannin and polyphenol contents of seed and pericarp of three Coffea species

Objective: To investigate the antioxidant activity, total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis (C. benghalensis) and Coffea liberica compared to Coffea arabica (C. arabica). Methods: The antioxidant potential, total tannin and polyphenol contents of the immature and mature seed and pericarp of C. benghalensis and Coffea liberica were quantified and compared to C. arabica. Enhanced chemiluminescence (ECL), 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity, Folin-Ciocalteau method and total tannin content assays were used. Results: Trolox equivalent (TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation (r